Fig. 3.

Pharmacokinetic profiles of H-Zt/g4-MMAE in both mouse and cynomolgus monkey: (a) PK profiles of H-Zt/g4-MMAE in mouse. Tumor-bearing and -nonbearing mice (athymic nude, 5 mice per group) were injected with a single dose of H-Zt/g4-MMAE at 3, 10, and 20 mg/kg, respectively. Collected blood samples were analyzed using the MMAE ADC ELISA kit (Eagle Biosciences, Inc., Nashua, NH). Various PK parameters were calculated using the software provided by Eagle Biosciences. (b) Free MMAE dissociated from H-Zt/g4-MMAE in monkey plasma. A single dose of H-Zt/g4-MMAE at 10 or 30 mg/kg was injected into cynomolgus monkey (3 animals per group). Free MMAE from individual blood samples collected at different time intervals were subjected to the LC-MS/MS analysis. (c) PK profiles of H-Zt/g4-MMAE in cynomolgus monkey. Blood samples from (b) were analyzed for MMAE coupled H-Zt/g4 using the MMAE ADC ELISA kit as described in (a) to obtain various PK parameters