Fig. 1.

LPS-induced NF-κB p50 DNA binding changes across the mouse lifespan. NF-κB p50 activity was assessed by determining NF-κB p50 DNA binding in whole brain nuclear extract from 9-month-old and 3-week-old C57BL/6 mice injected with LPS (5 mg/kg, IP). Whole brain nuclear extract from the older mice injected with LPS showed reduced DNA binding when compared to the younger mice. Values are reported as mean percent of 3 week saline control ± SEM. An asterisk indicates significant difference (P < 0.05) from control. n = 4