Fig. 5.

Representative images demonstrating augmented changes in microglia morphology in middle-aged NF-κB p50^−/− mice. Young adult (1.5–3.0 month old) and middle-aged (16.0–18.0 month old) NF-κB p50^+/+ and NF-κB p50^−/− mice were injected with saline or LPS (5 mg/kg, IP) to evaluate loss of NF-κB p50 function on microglia morphology in vivo. Three coronal sections (40 μm) per animal containing the substantia nigra pars compacta were stained with IBA1. Representative images are shown from the substantia nigra pars compacta at × 40 and the scale bar depicts 50 μM. n = 3