Fig. 7.

NF-κB p65 DNA expression and activity in aging and NF-κBp50^−/− mice. Young adult (1.5–3.0 month old) and middle-aged (16.0–18.0 month old) NF-κB p50^+/+ and NF-κB p50^−/− mice were injected with saline or LPS (5 mg/kg, IP). Nuclear extract collected from whole brain tissue at 3 h following a saline or b LPS injection was evaluated for NF-κB p65 DNA binding with ELISA. c Total NF-κB p65 protein in brain tissue at 3 h post-injection was evaluated by western blot and normalized to GAPDH. d Representative image of NF-κB p65 and GAPDH western blot bands used to calculate the total NF-κB p65 brain protein. Values are reported as mean percent of saline control ± SEM. An asterisk indicates a significant age effect (P < 0.05) and a dagger indicates a significant difference between mouse strains (P < 0.05). n = 3