Table 4.

IL-1β mRNA expression in microglia isolated from saline-treated control mice

Group	IL-1β	SEM	P value
Young p50+/+	100.00	38.00
Young p50−/−	95.93	24.00
Middle-aged p50+/+	100.00	12.30
Middle-aged p50−/−	120.00	57.40

Young adult (1.5–3.0 month old) and middle-aged (16.0–18.0 month old) NF-κB p50^+/+ and NF-κB p50^−/− mice were injected with saline, and isolation of microglia from the whole brain with CD11b microbeads was performed at 3 h post-injection. IL-1β mRNA levels were evaluated by quantitative RT-PCR. Values are normalized to GAPDH using the 2^-ΔΔCT method. *Significant difference (P < 0.05) between mouse strains. n = 3