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. 2019 Mar 12;13:23. doi: 10.1186/s13036-019-0152-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Visualization of the cleavage events a and b to produce A₁D₃. Pictured here are the most common PA of A₁D₃ found in the hydrolysates produced with CSN, CSN-Y270A, CSN-E59A, and CSN-W118A from Fig. 7. The positioning of the GlcN/GlcNAc units at the different subsites is shown. Also indicated by black triangles are the positions at which the two cleavage events must have occurred to produce the oligomer