Figure 3.

A schematic diagram of cyp51A gene showing the position and composition of 34-bp tandem repeat loop-mediated isothermal amplification (TR34-LAMP) primers for amplification of the tandem repeat region. Forward outer primer (F3), backward outer primer (B3), forward inner primer FIP (F1c+F2), backward inner primer BIP (B1c+B2), loop forward primer (LF). Loop backward primer is not included in this assay due to design restrictions. F2 primer is placed within the first tandem repeat and F1c is located at the downstream of second tandem repeat to avoid self-annealing of FIP. LF primer is located in the junction of the two tandem repeats. The rest of the LAMP primers are outside of tandem repeat region.