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. 2019 Mar;21(2):286–295. doi: 10.1016/j.jmoldx.2018.10.004

Figure 4.

Figure 4

Real-time 34-bp tandem repeat loop-mediated isothermal amplification (TR34-LAMP) amplification plot and standard curve. A: Detection of different concentrations of synthetic TR34 DNA. As low as 10 copies/reaction were detected within 22 minutes. B: Standard curve for TR34-LAMP assay generated from the amplification plot between serial 10-fold dilutions of TR34 synthetic DNA. Nine replicates per concentration were used. Nontemplate controls (NTC) were also included, which remained negative for 30 minutes. Data are expressed as means ± SD.