Table 2.
Validation of the TR34-LAMP Assay with Clinical Isolates
| Type | Clinical sample |
A. fumigatus detection |
TR34 mutation detection |
|||
|---|---|---|---|---|---|---|
| LAMP A. fumigatus∗ |
TTP, means ± SD, minutes | TR34-LAMP† | TTP, means ± SD, minutes | Estimated concentration‡ | ||
| TR34 | CL-Asp164 | + | 23.19 ± 0.25 | + | 16.45 ± 0.31 | 7.38 × 103 |
| CL-Asp168 | + | 23.29 ± 0.50 | + | 15.85 ± 0.17 | 1.38 × 104 | |
| CL-Asp251 | + | 24.6 ± 1.14 | + | 16.03 ± 0.13 | 1.14 × 104 | |
| E076 | + | 22.23 ± 0.28 | + | 15.92 ± 0.20 | 1.28 × 104 | |
| TR46 | E224 | + | 22.82 ± 0.27 | − | − | − |
| E357 | + | 21.32 ± 0.29 | − | − | − | |
| E276 | + | 22.55 ± 0.23 | − | − | − | |
| WT | E409 | + | 25.82 ± 0.42 | − | − | − |
| ARAF016 | + | 22.72 ± 0.21 | − | − | − | |
| AF293 | + | 24.98 ± 0.81 | − | − | − | |
| CL-ASP237 | + | 22.39 ± 0.14 | − | − | − | |
SDs were calculated from replicates.
+, positive reaction; −, negative reaction; A. fumigatus, Aspergillus fumigatus; LAMP, loop-mediated isothermal amplification; TTP, time to positivity (detection within 30-minute timeframe); WT, wild type.
∗
LAMP A. fumigatus, result of LAMP assay by using published LAMP primers43 for A. fumigatus detection.
†
TR34-LAMP, result of TR34-LAMP assay by using clinical samples.
‡
Estimation of initial concentration (Copy number/Reaction) by linear regression formula [y = −0.96ln(x) + 24.96].