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. 2019 Mar 8;13:30. doi: 10.3389/fnana.2019.00030

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Copyright © 2019 Haidar, Tin, Zhang, Nategh, Covita, Wykes, Rogers and Gundlach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparative distribution of relaxin-3-immunoreactive nerve fibers and neurons positive for GABAergic and cholinergic markers in the MS/DB of adult mice. (A) A mosaic digital image (20× images represented as z-stack maximum projections, 1 μm intervals) of mouse MS/DB at 0.74 mm from bregma, illustrating relaxin-3 (green), ChAT (red) and PV (blue) immunoreactivity. (B,C) High-resolution images (63×, z-stack maximum projections, 0.1 μm intervals) of the boxed areas in (A), illustrate the potential close association of relaxin-3 elements with a ChAT-immunoreactive neuron in MS (B,B′) and a PV-immunoreactive neuron in horizontal DB (C,C′). Scale bars 200 μm (A), 20 μm (B,C) and 5 μm (B′,C′).