Table 2.
HNC patients, paired oral samples, and longitudinal PERMANOVA analyses.
|
Longitudinal Analysis T0-Tx |
Patient Count (M/F) | Patient %M/F | Paired Samples | PERMANOVA Time: All Probes* | PERMANOVA Time: Caries Probes** |
|---|---|---|---|---|---|
| Set-1 [T0-T6] | 31 (26/5) | 84/16 | 101 | 0.0001 | 0.0001 |
| Set-1 [T0-T18] | 23 (19/4) | 83/17 | 69 | 0.0003 | 0.0001 |
| Set-2 [T0-T6] | 22 (18/4) | 82/18 | 60 | 0.0002 | 0.0004 |
| Set-2 [T0-T18] | 22 (18/4) | 82/18 | 60 | 0.0005 | 0.0003 |
| Subset-A [T0-T6] | 28 (23/5) | 82/18 | 90 | 0.0001 | 0.0001 |
| Subset-A [T0-T18] | 20 (16/4) | 80/20 | 58 | 0.0002 | 0.0001 |
| Subset-B [T0-T6] | 20 (16/4) | 80/20 | 63 | 0.0001 | 0.0001 |
| Subset-B [T0-T18] | 14 (11/3) | 79/21 | 39 | 0.0038 | 0.0033 |
| Subset-C [T0-T6] | 21 (17/4) | 81/19 | 66 | 0.0001 | 0.0001 |
| Subset-C [T0-T18] | 14 (11/3) | 79/21 | 38 | 0.0017 | 0.0007 |
| Subset-D [T0-T6] | 24 (20/4) | 83/17 | 79 | 0.0001 | 0.0001 |
| Subset-D [T0-T18] | 19 (15/4) | 79/21 | 55 | 0.0001 | 0.0001 |
Longitudinal multivariate permutational analyses of variance (PERMANOVA) were performed on square root transformed relative abundance data. Monte-Carlo corrected p-values for the fixed variable ‘Time’ are shown with alpha level of significance level set at 0.01.
Set-1: all patients with and without DMFS score information
Set-2: all patients samples in common across the three timepoints T0, T6, and T18, with and without DMFS score information.
Set-1 subsets of patients with DMFS score information: Subset-A (all patients), Subset-B (all patients who did not receive induction chemotherapy), Subset-C (all patients who did not receive antibiotics within 2 weeks of sampling), Subset-D (all patients who received concurrent chemotherapy).
T0 to Tx: baseline to 6-months post-RT sampling (T0-T6), or baseline to 18-months post-RT sampling (T0-T18).
Gender: M (male), F (female).
*Species probes (n = 638) plus genus probes (n = 129); **probes for caries-associated species (n = 45).