Table 1.
Effects of modification of COMT and F5H on biomass, cell wall digestibility and lignin content of transgenic switchgrass plants
| Dry matter biomass (g/plant) | Enzymatic hydrolysis efficiency (%) | Acetyl bromide lignin (mg/g CWR) | |
|---|---|---|---|
| Control | 21.7 ± 1.5a | 32.2 ± 0.9e | 234.6 ± 3.4c |
| COMTRi1 | 20.5 ± 3.9a | 40.7 ± 1.1c | 217.5 ± 1.6b |
| COMTRi2 | 23.5 ± 1.5a | 36.4 ± 0.4d | 230.1 ± 2.4c |
| F5HRi‐1 | 20.9 ± 2.2a | 33.4 ± 0.7ed | 224.8 ± 4.9c |
| F5HOE‐1 | 22.4 ± 2.1a | 32.5 ± 1.0ed | 240.5 ± 3.6c |
| COMTRi1_F5HRi‐1 | 21.3 ± 4.3a | 51.1 ± 0.9a | 195.8 ± 5.3a |
| COMTRi1_F5HRi‐2 | 20.3 ± 3.1a | 50.5 ± 0.8a | 188.8 ± 4.4a |
| COMTRi1_F5HRi‐3 | 18.8 ± 2.1a | 51.2 ± 1.4a | 187.2 ± 1.5a |
| COMTRi1_F5HOE‐1 | 23.1 ± 3.3a | 44.1 ± 1.3bc | 219.6 ± 1.9b |
| COMTRi1_F5HOE‐2 | 17.3 ± 3.6a | 45.4 ± 0.5b | 208.1 ± 0.7ab |
| COMTRi1_F5HOE‐3 | 19.5 ± 2.0a | 43.0 ± 1.9bc | 215.4 ± 1.9b |
| COMTRi2_F5HOE‐1 | 20.6 ± 2.9a | 30.8 ± 0.9e | 232.6 ± 4.3c |
| COMTRi2_F5HOE‐2 | 21.9 ± 1.6a | 31.9 ± 2.9e | 232.7 ± 1.0c |
| COMTRi2_F5HOE‐3 | 24.2 ± 3.8a | 32.1 ± 0.8e | 225.4 ± 3.7c |
The transgenic and control plants were harvested after six months of growth in the greenhouse. Values are mean ± SE (n = 3). Means with the same letter are not significantly different (One‐way ANOVA, Duncan's test, P < 0.05).