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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: J Vis Exp. 2019 Jan 7;(143):10.3791/58647. doi: 10.3791/58647

Figure 1: Deoxygenation chamber.

Figure 1:

Mixture of PRP and RBCs is added into a plastic bottle closed with a rubber septum. The closed bottle is connected to helium (He) line using a needle. Oxygen is flushed out by a small syringe needle (26 G) serving as a gas outlet.