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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: J Vis Exp. 2019 Jan 7;(143):10.3791/58647. doi: 10.3791/58647

Figure 5: VASPSer239 phosphorylation depends on pO2 and hematocrit levels.

Figure 5:

(A) PRP + RBCs (20% hematocrit) were deoxygenated to two different pO2 levels, 40 and 25 mmHg. P-VASPSer239 and VASP were measured after the incubation of deoxygenated samples with 10 M nitrite at 37 C for 10 min. (B) Nitrite (10 M) was added into deoxygenated PRP + RBCs at 0, 10, 20 and 40% hematocrit (pO2 = 25 mmHg). Fold increased (P-VASPSer239) was calculated relative to control (PRP+RBCs without nitrite) for each hematocrit value. Error bars = SEM. Please click here to view a larger version of this figure.