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Effects of the NKCC1 inhibitor bumetanide injected into the SCN on the phase of the circadian locomotor rhythm in the early subjective night (CT 13.5). Top: Bumetanide did not produce phase shifts in the free-running locomotor rhythm at any concentration (p > 0.05). Middle and bottom: Representative wheel-running actograms for vehicle injection and 10 μM bumetanide injections, respectively. Gray boxes denote manipulation time point (i.e., drug treatment).
