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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Methods Cell Biol. 2017 Nov 26;143:261–279. doi: 10.1016/bs.mcb.2017.08.015

Figure 1.

Figure 1.

SDS-PAGE analysis of total extract (lanes 1 and 4), DEAE unbound (lanes 2 and 5), and the 2 M NaCl fraction of total proteoglycans (lanes 3 and 6). Lanes 1–3, intact. Lanes 4–6, Chondroitinase ABC digested. The gel was stained with Coomassie blue and Alcian blue. Intact versican (indicted with bracket) remains in the stacking gel and at the top of the running gel. Putative biglycan and decorin are indicated with asterisks. Following digestion with chondroitinase, the core proteins for versican V0 and V1 appear at about 550 and 500 kDa. Biglycan and decorin core proteins and enzyme bands are indicated.