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. 2019 Mar 15;6:3. doi: 10.1186/s40694-019-0066-9

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Schematic presentation of the a workflow and b target genes for multiplexed A. niger genome editing using the microtiter plate scale CRISPR/Cas9 method. The workflow was demonstrated by engineering the fungal d-galacturonate pathway for galactarate (mucic acid) production. The competing pathway genes gaaA, encoding a d-galacturonate reductase, gaaC, encoding an l-galactonate dehydratase, and 39114, encoding an enzyme involved in galactarate catabolism, and gaaX, encoding a repressor protein of pectin catabolic pathways, were deleted by replacing the genes with DNA cassettes expressing the bacterial uronate dehydrogenase (UDH)