Table 2.

Primer list for PCR of BSgDNA

Locus	1st PCR primer pair (5′-3′)	2nd PCR primer air (5′-3′)
IGF2-H19 ICR	F–AGGTGTTTTAGTTTTATGGATGATGG [47] R–TCCCATAAATATCCTATTCCCAAATAACC [47]	F–TGTATAGTATATGGGTATTTTTGGAGGTTT [47] R–TCCCATAAATATCCTATTCCCAAATAACC [47]
DLK1-MEG3 IG-DMR (CG6)	F–TGGGAATTGGGGTATTGTTTATAT R–AAACAATTTAACAACAACTTTCCTC	F–GTTAAGAGTTTGTGGATTTGTGAGAAATG [39] R–CTAAAAATCACCAAAACCCATAAAATCAC [39]
MEG3 DMR (CG7)	F–TTATTTTTTTGAATAATAAGAGAAAGTATG R–CTCATTTCTCTAAAAATAATTAACC	F–TTATTTTTTTGAATAATAAGAGAAAGTATG R–CCCCAAATTCTATAACAAATTACT [39]
MEG3 promotor (CG9)	F–TGAGGAAGTAGGGGTTTATAGAGAG R–AACCCTACAACCCCACAAAA [39]	F–GGAGAGTGGGGTTTATTGTGAA [39] R–AACCCTACAACCCCACAAAA [39]
MEG3 intragenic (CG8)	F–GTTTGAGATTTGTTGGGTATTT [39] R–AATTTAACTAACAAATCACAAATATTAACT	F–GTTTGAGATTTGTTGGGTATTT [39] R–AATTTAACTAACAAATCACAAATATTAACT
ZAC DMR	F–GGGGTAGTYGTGTTTATAGTTTAGTA [48] R–CRAACACCCAAACACCTACCCTA [48]	F–GGGGTAGTYGTGTTTATAGTTTAGTA [48] R–CRAACACCCAAACACCTACCCTA [48]
PEG1 DMR	F–TTGTTGGTTAGTTTTGTAYGGTT [47] R–AAAAATAACACCCCCTCCTCAAAT [47]	F–TTGTTGGTTAGTTTTGTAYGGTT [47] R–CCCAAAAACAACCCCAACTC [47]
PEG3 DMR	F–AAAAGGTATTAATTATTTATAGTTTGGT [49] R–AAAAATATCCACCCTAAACTAATAA [49]	F–AAAAGGTATTAATTATTTATAGTTTGGT [49] R–AAAAATATCCACCCTAAACTAATAA [49]