Fig. 3. Panel (A): sensograms (thin lines) and fitted curves (bold lines) of ubiquitin interacting with immobilized Aβ40. Five concentrations of ubiquitin were injected: 43 μM (green lines), 86 μM (blue lines), 171 μM (red lines), 343 μM (black lines), and 755 μM (orange lines). Panel (B): MST traces (left) of titrations of Aβ40 against ubiquitin; F0 (blue bar) and F1 (red bar) correspond to the fluorescence of unbound state and bound state, respectively. Plot of normalized fluorescence (right) obtained from Ub binding experiments versus Aβ40 at different concentrations (from 20 nM to 1 mM).
