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. 2019 Mar 15;15(3):e1008005. doi: 10.1371/journal.pgen.1008005

Fig 7. CRISPR/Cas9 knock-in of the Gal4 gene into the Or22a and Or22b coding regions.

Fig 7

(A) Genomic region of Or22a and Or22b before and after it is targeted for CRISPR/Cas9-mediated homology-directed repair to knock-in the Gal4 transcription factor and DsRed eye marker genes. The formal designation of this stock is Df(2L)Or22ab, TI{GAL4}Or22ab but for convenience it is indicated as Or22abGAL4. (B) GFP signal from antennal cross sections from D. melanogaster carrying the Gal4 knock-in (Or22abGAL4), the UAS-mcd8:GFP transgene on the second chromosome, or both. (C, D) Odorant response profiles from (C) wild type control ab3A and (D) homozygous Or22abGAL4 ab3A ORNs. (E, F) Odorant response profiles from D. melanogaster UAS-Or transgenes previously shown [15] to confer strong responses to E2-hexenal (Or7a) (E) or methyl salicylate (Or10a) (F), expressed in homozygous Or22abGAL4 ab3A olfactory receptor neurons. In panels C-F neuronal responses are reported in spikes/second +/- S.E.M., using individual odorants diluted at 10−2 and presented for 0.5 seconds. n≥5 for all odorants tested. Spontaneous firing frequencies have been subtracted from all responses; responses to the paraffin oil diluent have been subtracted from the responses to all odorants.