Figure 1.

Hypoxic NSCLC cell-derived exosomes reduced the sensitivity of normoxic tumor cell to cisplatin.

Notes: (A) The upregulation of HIF-1α in CoCl₂-treated NSCLC cells as verified by Western blot. (B) The enrichment of CD63 and ALIX in exosomes (exo) purified from SK-MES-1 cell-conditioned medium and A549 cell-conditioned medium as determined by Western blot. (C) SK-MES-1 cell-derived and A549 cell-derived exosomes showed the typical morphology and size (50–150 nm) based on electron micrographs, bar =100 nm. (D) Effects of 30 µM cisplatin on the vitality of hypoxia-induced exosomes treated with NSCLC cells as assessed by MTT assay. (E) Effects of 2 µM cisplatin on colony formation of hypoxia-induced exosomes treated with NSCLC cells. All experiments were performed in triplicate. Values are presented as mean ± SD. Error bars, SD. *P<0.05.

Abbreviations: CoCl₂, Cobalt (II) chloride; HIF-1α, hypoxia-inducible factor-1α; NSCLC, non-small-cell lung cancer.