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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Immunol Lett. 2016 Sep 14;179:85–94. doi: 10.1016/j.imlet.2016.09.006

Fig. 6.

Fig. 6

Cross-reactivity of polyclonal antibody and antisera against recombinant mouse OPN. Both animal models were fed either a DDC or HF and NC or LF diet for 17 and 14 weeks, respectively (n = 14–15 per group). Immunization was performed using scrambled control OPN-peptides (Control pep 1 and 2; Ctrl 1 and 2), different OPN-derived peptides (Peptide 1–4; Pep 1–4) and Adj-KLH, as indicated. Mean titer of polyclonal antibody (pAB) titration of the cardio-metabolic (Ldlr−/− mice) (A) and the DIO model (WT mice) (B) as well as possible cross-reactivity of obtained end-sera from Ldlr−/− (C) and WT mice (D) against recombinant mouse OPN was determined with ELISA. Data is expressed as mean.