Fig. 3.

Homoplasmy of transplastomic lines obtained with vectors pCH8, pJF1153 and pJF1151, and demonstration of maternal transgene inheritance. The left plates show the comparison between the progenies of the selfed transplastomic line (Tp) and the selfed At-Δacc2 recipient line used for transformation. The right plates show the progenies of reciprocal crosses between the wild type (Wt) and the transplastomic line. Seeds were germinated in the presence of 100 mg/L spectinomycin. These assays were repeated independently two times with similar results.