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. Author manuscript; available in PMC: 2019 Aug 18.
Published in final edited form as: Nat Plants. 2019 Feb 18;5(3):282–289. doi: 10.1038/s41477-019-0359-2

Fig. 4.

Fig. 4

Expression of the YFP reporter in transplastomic At-Δa-JF1151 plants. (a) Chlorophyll and YFP fluorescence of wild-type (Wt) and transplastomic (Tp) seedlings grown in the absence (Spec0) or presence (Spec100) of spectinomycin. The plates were scanned with a Typhoon imager that produces a red image of the chlorophyll fluorescence and a green image for the (yellow) YFP fluorescence. The images were taken 20 days after sowing. (b) Confirmation of chloroplast YFP expression in leaf mesophyll cells by confocal laser-scanning microscopy. BF: bright-field image; Chl: chlorophyll fluorescence; YFP: YFP fluorescence (colored in green, to match the color of the Typhoon image); Chl+YFP: overlay of the chlorophyll and YFP fluorescences. These experiments were repeated independently three times with similar results.