Fig. 5.

Immunoblot analysis of YFP accumulation in transplastomic Arabidopsis plants. Two independently generated transplastomic At-Δa-JF1151 lines were analyzed. The wild type (At-Wt) and a transplastomic At-Δa-CH8 line were included as negative controls. Samples of total soluble protein extracted from leaves (with the amounts given in µg) were separated by polyacrylamide gel electrophoresis and blotted. A dilution series of YFP purified from Escherichia coli (with the amounts given in ng) was loaded for semiquantitative assessment of protein accumulation in transplastomic plants. Note that the YFP recombinantly expressed in bacteria migrates slightly slower than the YFP in the transplastomic samples. This is due to the presence of a purification tag (His-tag) in the YFP isolated from E. coli. See Methods for further details. These experiments were repeated independently two times with similar results.