Table 1.
Summary of transformation experiments conducted in Arabidopsis thaliana. Nt: Nicotiana tabacum; P: promoter; T: 3’ UTR; G10: gene10 leader from bacteriophage T7; Cr: Chlamydomonas reinhardtii; Zm: Zea mays; Ec: Escherichia coli.
| Vector | Transgene cassettes | Selection | Recipient | Bombarded plates | Resistant lines | Resistant lines analyzed | Confirmed transplastomic lines | Spontaneous mutants | Lines grown to maturity | Lines with seeds obtained |
|---|---|---|---|---|---|---|---|---|---|---|
| pSTS5 | NtPrrn:rbcL::aadA::NtTpsbA | spec | WT | 60 | 0 | 0 | 0 | 0 | ||
| pSTS7 | NtPrrn:G10::aadA::NtTpsbA | spec | WT | 155 | 0 | 0 | 0 | 0 | ||
| pSTS10 | NtPrrn:rbcL::aadA::NtTpsbA CrPrrnG10:gfp::CrTatpA | spec | WT | 92 | 0 | 0 | 0 | 0 | ||
| pSTS12 | NtPrrn:G10::aadA::NtTpsbA CrPrrnG10:gfp::CrTatpA | spec | WT | 90 | 1 | 1 | 0 | 1 | ||
| pSTS13 | NtPrrn:G10::aphA-6::NtTpsbA CrPrrnG10:gfp::CrTatpA | kan | WT | 192 | 0 | 0 | 0 | 0 | ||
| pCH7 | ZmPclpP:G10::aphA-6::NtTrps16 | kan | WT | 259 | 0 | 0 | 0 | 0 | ||
| pCH8 | ZmPclpP:G10::aadA::NtTrps16 | spec | WT | 507 | 1 | 1 | 1 | 0 | 1 | 1 |
| pCH8 | ZmPclpP:G10::aadA::NtTrps16 | spec | Δacc2 | 98 | 43 | 30 | 29 | 1 | 8 | 6 |
| pJF1153 | ZmPclpP:G10::synaadA::EcTrrnB | spec | Δacc2 | 141 | 43 | 34 | 30 | 4 | 14 | 12 |
| pJF1151 | ZmPclpP:G10::synaadA::EcTrrnB CrPpsbA::yfp::CrTatpB | spec | Δacc2 | 18 | 6 | 5 | 5 | 0 | 3 | 3 |