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. Author manuscript; available in PMC: 2019 Jul 28.
Published in final edited form as: Nat Med. 2019 Jan 28;25(3):496–506. doi: 10.1038/s41591-018-0336-8

Figure 4. ApoE or ApoE139–152 binds to the C1q stalk.

Figure 4

(a) Four peptides are depicted in a 3D model of human ApoE3 (PDB ID code: 2L7B) and their corresponding amino acid sequences. (b) ApoE4 inhibition was blocked by ApoE peptide P139–152 but not by ApoE peptides P30–40, P74-85, P210–232. (c) Binding of ApoE isoforms and corresponding ApoE peptides to C1q were determined by ELISA. (d) Binding affinity of P139-152 to C1q was determined by MicroScale initial fluorescence analysis. (e) Binding of ApoE3 to C1q and LDLR in the presence of SDS or NaCl was determined by ELISA. (f) ApoE binds to the stalk of C1q. C1q alone or incubated with biotinylated plasma-purified ApoE3 or biotinylated ApoE peptide P139-152 and streptavidin-coated gold particles were visualized by electron microscopy. Representative image from 3 independent experiments. Gold-streptavidin/biotin-ApoE and recombinant directly gold-labeled ApoE showed similar results. Bar 20 nm. Data represent means ± SEM of three independent experiments. Two-tailed Student´s t-test.