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. Author manuscript; available in PMC: 2019 Jul 28.
Published in final edited form as: Nat Med. 2019 Jan 28;25(3):496–506. doi: 10.1038/s41591-018-0336-8

Extended Figure 2. Complement constituents in mouse ChPs.

Extended Figure 2

(a) ChPs were stained for C1q (red) and C4 (green). Bar 100 µm. (b) C5 siRNA treatment blocks C5 protein deposition in ApoE-/- ChPs; (c) ChPs were stained for C3. Ig represents lipid; (d) Serum C3 and C5. Serum C3 and C5 protein levels were measured by ELISA. ApoE-/-(n = 6 mice), HFD ApoE4 (n=5). (e) High resolution confocal microscopy shows colocalization of ApoE4 (ApoE, red) and Ig (green, represents lipid) in HFD ApoE4-KI ChPs. ApoE-/- ChPs serve as negative controls for ApoE staining; (f) Complement regulators are expressed in ChPs. WT (n = 5 mice); ApoE-/-(n=4); ND ApoE3 (n=6); HFD ApoE3 (n=6); ND ApoE4 (n=6); HFD ApoE4 (n=6). (g) ChP Factor H expressed between WT and ApoE-/- mice. WT (n=5); ApoE-/-(n=4); (h) ChP factor H protein in ChPs. White arrows indicate lipid positive areas. Data in a,b,c,e,h are representative images from at least 3 biologically independent mouse samples. Data in d,f,g represent means ± SEM; Two-tailed Student's t-test was applied to d,g; one-way ANOVA with Tukey posttest was applied to f; Gene names in Supplementary Table 3.