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. 2019 Jan 7;29(3):221–232. doi: 10.1038/s41422-018-0128-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — TUT4-deficient and TUT7-deficient animals are fertile but combined TUT4/7-deficient animals show growth retardation and die perinatally. a Pups per litter for Tut4/7^CTL, Tut4^−/−, and Tut7^−/− males are shown. Each dot corresponds to a single litter. The center value represents the mean, and the error bars the standard deviation. (ns not significant; Wilcoxon test two-sided; Tut4/7^CTL, litters = 8, sires = 3; Tut4^−/−, litters = 6, sires = 3; Tut7^−/−, litters = 6, sires = 3). b Testis weight for Tut4/7^CTL, Tut4^−/−, and Tut7^−/– animals. Each dot corresponds to a single testis. The center value represents the mean, and the error bars the standard deviation. (****P < 0.0001, Wilcoxon test two-sided; Tut4/7^CTL, n = 50; Tut4^−/−, n = 28; Tut7^−/−, n = 24). c Body weight for Tut4/7^CTL, Tut4^−/−, and Tut7^−/− males. Each dot corresponds to the body weight of a single animal. The center value represents the mean, and the error bars the standard deviation. (ns not significant; *P < 0.05, Wilcoxon test two-sided; Tut4/7^CTL, n = 25; Tut4^−/−, n = 14; Tut7^−/−, n = 12). d Ratio of testes to body weight for Tut4/7^CTL, Tut4^−/−, and Tut7^−/− males. Each dot corresponds to the testes/body weight ratio of a single animal. The center value represents the mean, and the error bars the standard deviation. (**P < 0.05, ***P < 0.01, ****P < 0.001, Wilcoxon test two-sided). e Micrographs of PAS-stained tubule sections from Tut4/7^CTL, Tut4^−/−, and Tut7^−/− mice. Scale bar, 20 μm. f Table of pups/embryos observed for different genotypes at different stages of development (weaned, E7.5-10.5, E11.5-14.5, and E15.5-E17.5). The expected numbers of TUT4/7-deficient animals are shown in brackets. g Pictures of P0 Tut4/7^CTL and Tut4/7^−/− animals are shown. Objective magnification, 1 × . h Pictures of Tut4/7^CTL and Tut4/7^−/− mice at embryonic stages E11.5, E13.5, E15.5, and E17.5 are shown. Objective magnification, 2× for E11.5 and E13.5 embryos and 1× for E15.5 and E17.5 embryos. i Western blots of extracts from WT, Tut4^{HA-GFP/HA-GFP} and Tut7^{HA-GFP/HA-GFP} embryos at different stages of development (E10.5 to E17.5) using anti-HA and anti-Tubulin antibodies. j H&E stained sections of Tut4/7^CTL and Tut4/7^−/− animals at E13.5 and E15.5 embryonic stages are shown. Objective magnification, 10×