Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila
melanogaster) |
AGO1 | NA | FLYB: FBgn0262739 | |
| Gene (D. melanogaster) | RpL32 | NA | FLYB: FBgn0002626 | |
| Gene (D. melanogaster) | eEF5 | NA | FLYB: FBgn0285952 | |
| Gene (D. melanogaster) | Moonshiner | NA | FLYB: FBgn0030373 | |
| Strain, strain background (D. melanogaster) |
w1118 | Laboratory Stock | FLYB: FBal0018186 | |
| Strain, strain background (D. melanogaster) |
BX2 |
Dorer and Henikoff, 1994
PMID:8020105 |
FLYB: FBti0016766 | |
| Strain, strain background (D. melanogaster) |
P(TARGET)GS | Bloomington Drosophila Stock Center |
FLYB: FBst0011039 | also called P-1039 |
| Strain, strain background (D. melanogaster) |
P(TARGET)G | Bloomington Drosophila Stock Center |
FLYB: FBti0003435 | also called BQ16 |
| Strain, strain background (D. melanogaster) |
P(TARGET)S | Bloomington Drosophila Stock Center |
FLYB: FBti0003418 | also called BA37 |
| Strain, strain background (D. melanogaster) |
nosGAL4 | Bloomington Drosophila Stock Center |
FLYB: FBti0131635, RRID:BDSC_32180 |
|
| Genetic reagent (D. melanogaster) |
P(lacW) | PMID: 2558049 | FLYB: FBtp0000204 | |
| Genetic reagent (D. melanogaster) |
P(PZ) |
Mlodzik and Hiromi, 1992
doi: 10.1016/B978-0-12-185267-2.50030–1 |
FLYB: FBtp0000210 | |
| Genetic reagent (D. melanogaster) |
P(A92) | PMID: 2827169 | FLYB: FBtp0000154 | |
| Genetic reagent (D. melanogaster) |
Moon shRNA PA61 |
Andersen et al., 2017
doi:10.1038/nature23482 |
Dr. Julius Brennecke (Institute of Molecular Biotechnology, Vienna) |
|
| Genetic reagent (D. melanogaster) |
Moon shRNA PA62 |
Andersen et al., 2017
doi:10.1038/nature23482 |
Dr. Julius Brennecke (Institute of Molecular Biotechnology, Vienna) |
|
| Antibody | Mouse IgG polyclonal antibody |
Merck (ex-Millipore) | Cat# 12-371B, RRID:AB_2617156 |
|
| Antibody | Rabbit polyclonal antibody against H3K9me3 |
Merck (ex-Millipore) | Cat# 07–442 | |
| Antibody | Rabbit polyclonal antibody against Rhino |
PMID: 19732946 | Dr. William Theurkauf (University of Massachusetts Medical School, Worcester) |
|
| Sequence-based reagent |
RT-qPCR primers | Sigma-Aldrich | ||
| Sequence-based reagent |
RT-qPCR primers | Eurogentech | ||
| Commercial assay or kit |
RNeasy kit | Qiagen | Cat# 74104 | |
| Commercial assay or kit |
Illumina TruSeq Small RNA library preparation kits |
Fasteris | http://www.fasteris.com | |
| Commercial assay or kit |
Revertaid RT | Thermo Scientific | EP0442 | |
| Commercial assay or kit |
Random Hexamers | Invitrogen | N8080127 | |
| Commercial assay or kit |
DNaseI (Rnase free) | New Englands Biolabs | M0303S | |
| Commercial assay or kit |
dNTPs solution Mix | New Englands Biolabs | N0447S | |
| Commercial assay or kit |
Ribolock RNA inhibitor | Thermo Scientific | EO0381 | |
| Commercial assay or kit |
Ssofast Evagreen Supermix |
Biorad | Cat# 172–5204 | |
| Commercial assay or kit |
qPCR kit | Roche | Cat# 04887352001 | |
| Chemical compound, drug |
TRIzol | Invitrogen | Cat# 15596026 | |
| Chemical compound, drug |
Chloroform | Carlo Erba Reagents | Cat# 438601 | |
| Chemical compound, drug |
Chloroform | Sigma-Aldrich | C2432 | |
| Chemical compound, drug |
Ethanol (EtOH) | Merck millipore | Cat# 100983 | |
| Chemical compound, drug |
Ethanol (EtOH) | Honeywell | Cat# 32221 | |
| Chemical compound, drug |
Glycerol | VWR AnalaR NORMAPUR |
Cat# 24388.295 | |
| Chemical compound, drug |
Glutaraldehyde | Sigma Aldrich | G-5882 | |
| Chemical compound, drug |
Potassium hexacyanoferrate(III) |
Sigma Aldrich | P3667 | |
| Chemical compound, drug |
Potassium hexacyanoferrate(II) trihydrate |
Sigma Aldrich | P3289 | |
| Chemical compound, drug |
X-Gal | Dutscher | Cat# 895014 | |
| Chemical compound, drug |
NaCl | VWR AnalaR NORMAPUR | Cat# 27810.295 | |
| Chemical compound, drug |
NaCl | Sigma-Aldrich | Cat# 31432 | |
| Chemical compound, drug |
Formaldehyde | Sigma-Aldrich | Cat# 252549 | |
| Chemical compound, drug |
Schneider Medium | Gibco | Cat# 21720–024 | |
| Chemical compound, drug |
Insulin | Sigma-Aldrich | I4011 | |
| Chemical compound, drug |
PBS | Ambion | AM9625 | |
| Chemical compound, drug |
Triton | Sigma-Aldrich | T8787 | |
| Chemical compound, drug |
KCl | Ambion | AM9640G | |
| Chemical compound, drug |
HEPES | Fisher Scientific | BP299 | |
| Chemical compound, drug |
IPEGAL | Sigma-Aldrich | Cat# 18896 | |
| Chemical compound, drug |
DTT | Fisher Scientific | R0861 | |
| Chemical compound, drug |
Na Butyrate | Sigma-Aldrich | Cat# 07596 | |
| Chemical compound, drug |
EDTA free protease inhibitor |
Roche | Cat# 04693159001 | |
| Chemical compound, drug |
N lauryl sarkosyl | Sigma-Aldrich | L-5125 | |
| Chemical compound, drug |
BSA | Fisher Scientific | BP9703 | |
| Chemical compound, drug |
SDS 20% | Euromedex | EU0660-B | |
| Chemical compound, drug |
Tris HCl | Invitrogen | Cat# 15504–020 | |
| Chemical compound, drug |
Dynabeads A | Invitrogen | 10002D | |
| Chemical compound, drug |
Glycine | Sigma-Aldrich | G8898 | |
| Chemical compound, drug |
Isopropanol | VWR | Cat# 20842.298 | |
| Software, algorithm | Galaxy Server | ARTBIO | https://mississippi.snv.jussieu.fr/ | |
| Software, algorithm | Weblogo |
Crooks et al., 2004
doi:10.1101/gr.849004 |