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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Photochem Photobiol. 2018 Oct 14;95(2):605–611. doi: 10.1111/php.13021

Figure 3. Treatment of mice irradiated with UVA with baicalin results in an increase in IL-12 and IL-23 cytokines.

Figure 3.

Mice were sacrificed after 24hr post UVA exposure, single cell suspensions of skin (dermal/epidermal) were prepared from dorsal skin. Cells were counted and 2X106 cells per group were stimulated with PMA (50ng/ml) and ionomycin (250ng/ml) for 5 hr in the presence of brefeldin A (10ug/ml) for intracellular cytokine staining 3A-C. Cells were stained with anti-CD11b, anti-MHCII and anti-IL12p35, IL23p19 and IL12/23 p40 antibodies and analyzed by flow cytometry. A1-C1. There were significantly higher percentage IL-12p35, IL-23p19 and IL-12/23p40 cytokines (**p<0.01) in the skin of mice treated with baicalin than in vehicle treated mice. The histograms depict the mean ± SD of cell percentages per group. N=5; experiment was repeated twice.