Mice were sacrificed at the termination of the experiment and skin and tissue lysates were prepared from mice. The expression levels (A) MMP1, (B) MMP9, (C) IL-12p35, (D) IL-12/23 p40, (E) IL-1β, (F) IL-23p19 and IL10 were determined using RT PCR as described in Materials and Methods sections. Data are shown as the means ± SD from experiments with 5 animals in each group (**p < 0.01). N=5; experiment was repeated twice.