Fig. 5.
Linearity of starch decomposition by SoAMY alpha-amylase contained in crude media supernatants at prolonged incubation (up to 120 min). The test was conducted according to macroSNT methodology described in sections 2.3. and 2.3.1. using crude medium supernatants containing SoAMY alpha-amylase expressed and secreted by Po1g-derived Y. lipolytica strain, transformed with pYLSC-SoAMY genetic construct. The two curves correspond to the experimental results and the trend curve, provided with R2 coefficient, reflecting linearity of the reaction throughout the incubation time. X axis: time of incubation [min]; Y axis: absorbance values at 600 nm wavelength (Abs 600), reflecting degree of the copper ions reduction. Error bars indicate ±SD from triplicates