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. 2019 Jan 23;103(5):2367–2379. doi: 10.1007/s00253-018-09610-0

Fig. 6.

Fig. 6

Comparison of the assays performance in determination of amylolytic activities in crude medium supernatants of Y. lipolytica cultures. SoAMY alpha-amylase activity (a, c) determined via macro-/microSIT (a) and macro−/micro-SIT/SNT (c) assays in crude culture supernatants of Y. lipolytica strains; TlGAMY glucoamylase activity (b, d) determined via macro-/microSNT (b) and macro-/micro-SIT/SNT (d) assays in crude culture supernatants of Y. lipolytica strains; NC negative control, Po1g strain; PC positive control, Po1g-derivative bearing pYLSC-SoAMY (a, c) or pYLSC-TlGAMY (b, d) genetic construction; strains 1–5 AA – Po1d-derivatives bearing Golden Gate Assembly cassette (GGA)-SoAMY construction (a, c); strains 1-5GA – Po1d-derivatives bearing GGA-TlGAMY constrution (b, d); Cultures were conducted as described in section 2.2., and enzymatic assays—2.3. X axis: different crude supernatant preparations containing either SoAMY alpha-amylase (a, c) or TlGAMY glucoamylase (b, d) withdrawn from different Y. lipolytica strains culture; Y axis: activity values [U/mL] determined according to definitions given in sections 2.3.1. and 2.3.2 for SNT and SIT assay, respectively. Error bars indicate ±SD from triplicates. Statistical importance of the observed differences was analyzed as described in section 2.3.3; * denotes p < 0.05, ** p < 0.01, *** p < 0.005, NS not significant