Fig. 2.

Igsf21, Pde10a and Btbd3 expression in perinatal stages. Igsf21, Pde10a and Btbd3 expression in E17.5 embryos, sagittal (lateral to medial, a, b; d, e; g, h) and transversal (c, f, i) sections. Color-coded overlays of hybridizations for Igsf21 (red), Pde10a (green) and Btbd3 (blue) genes (j–l). a–cIgsf21 is strongly expressed in Po and DL nuclei. d–fPde10a is strongly labelled in the VM, VL and VPM. g–iBtbd3 displays a weak signal in VL, VPM, VPL and DL nuclei. j–l pseudo-colored superposition of adjacent sections depicting the complementary expression of the three genes. The molecular boundary between dorsal and ventral nuclei is clearly detectable, mainly between Igsf21 and Pde10a expression domains. Dashed lines in a, d and g correspond to the plane of the transversal sections (c, f, i). Scale bar: 200 µm