Fig. 3. Effects of decreased AID on tumourigenesis and metastasis of T24 cells in vivo.

a–c Suppressed AID expression clearly slowed down tumourigenesis, but no distinct tumour formation in nude mice injected with shAICDA-T24 cells was noted, as described above; n = 8. d, e Interfered AID significantly reduced the lung metastasis of tumour (P < 0.05). f, g Similar result detected by HE and IHC, respectively. Compared with that of nude mice injected with sh-AICDA-T24 cells, the pulmonary alveolus of nude mice in sh-Con group was destroyed more hardly by cancer nest; n = 8. Original magnification: c, f, g × 100 or × 200, calibration bar at 100 or 50 μm. All the experiments were repeated three times