Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The primer sequences used in MSP analysis
| Genes | Primers sequencing (5′ → 3′) | Primer pair (bp) | |
|---|---|---|---|
| H19(M) | Forward | GGATTTTGTTTTGCGGAAATCAC | 165 |
| Reverse | ATCACGACTCAAACCTCACG | ||
| Igf2(M) | Forward | AGGATTTCGTCGGGAGGCAC | 158 |
| Reverse | CACAAAATCCCGCACCCCG | ||
| H19(U) | Forward | GGGATTTTGTTTTGTGGAAATTAT | 167 |
| Reverse | ATCACAACTCAAACCTCACA | ||
| Igf2(U) | Forward | GTTTAGGATTTTGTTGGGAGGTAT | 162 |
| Reverse | CACAAAATCCCACACCCCA |
M, methylated; U, unmethylated
