Table 3.

Effect of biotin and pentoxifylline on the fertilization and embryo developmental potential in vitro

	Control	Biotin (10 nM)	PTX (1 mM)
Number of oocytes inseminated	291	366	358
Fertilization rate (%)	84.54	92.90 c	87.71 d
2-cell rate (%)	91.87	98.53 c	96.82 a
4-cell rate at 48 h post-insemination (%)	92.89	94.33	93.09
Compaction rate at 72 h post-insemination (%)	89.33	95.52 b	93.09
Blastocyst rate at 96 h post-insemination (%)	80.00	88.66 b	81.91 d
Blastocyst rate at 120 h post-insemination (%)	86.22	90.45	91.45
Hatching rate at 96 h post-insemination (%)	34.22	46.87 b	35.53 e
Hatching rate at 120 h post-insemination (%)	49.33	62.09 b	57.89
Total cell number (mean ± SE)	72.96 ± 3.63	91.05 ± 3.26 b	89.20 ± 3.08 b
Apoptotic index (mean ± SE)	6.12 ± 1.06	4.21 ± 0.38	4.07 ± 0.50

^aP < 0.05

^bP < 0.01

^cP < 0.001 vs control

^dP < 0.05

^eP < 0.01 vs biotin