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. 2019 Mar 15;10:1232. doi: 10.1038/s41467-019-09217-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Single-molecule imaging of PFF-1, rhodamine B (Rho B), and HMSiR in polymer films. a Examples of camera frames in which single molecules of PFF-1 were observed in the fluorescent state, and average intensity over all frames collected during a 10 s acquisition, color-coded according to time. Most spots in the color-coded image display multiple colors because single molecules emit photons at various time points. b Kymograph of a single molecule of probe PFF-1 switching for 10 s. c Kymograph of a single molecule of Rho B. No switching is observed, only irreversible transformation into a dark state. d Kymograph of a single molecule of probe HMSiR blinking spontaneously for 10 s. The chemical structure of each compound is displayed under the indicated panel. Scale bar in panel (a) 1 μm