Fig. 6. Heat shock inhibits peritonitis in mice.

C57BL/6 WT mice (5 animals per group) were heated on a pad (42 °C, 1 h) or not and left 24 h in their cage before being intraperitoneally injected with Alum (40 mg/mL) or MSU (10 mg/mL) for 6 h. IL-1β content in the lavage fluid was measured by ELISA (a) and the absolute number of neutrophils (CD11b/Gr1+) recruited was evaluated by flow cytometry (b). Insert: a representative western blot of HSP70 expression in peritoneal macrophages in mice. Data also represents the mean ± s.e.m. of three independent experiments. Statistics compare animals with or without heat shock: *p < 0.05; **p < 0.01; ****p < 0.0001; n.s. not significant using two tailed t test