Skip to main content
. 2019 Feb 18;7(1):31–45.

Figure 3.

Figure 3

IFIT5 regulates miR-363 turnover via recognition of precursor miRNA 5’end. A, B. The impact of IFNγ on the expression level of miRNAs derived from the miR-106a-363 cluster (miR-106a, miR-18b, miR-20b, miR-19b-2, miR-92a-2 and miR-363) in ACHN and 786O cells, compared to vehicle control (*P<0.05, ***P<0.0001). C. The impact of IFIT5 loss (shIFIT5) on the IFNγ-induced downregulation of miR-363 level in ACHN cells. (*P<0.05). D. Attenuated cancer invasiveness by miR-363 overexpression in 786O or ACHN cells, compared to vector control (*P<0.05, ***P<0.00001). E. Left: Mutation of nucleotides (box) for generating 5’-end 6 nucleotides single stranded pre-miR-363 (SS6Mut pre-miR-363) and blunt 5’-end double stranded pre-miR-363 (DSMut pre-miR-363). Both mature miR-363 and miR-363* sequence are shown in lighter gray. Right: Expression level of mature miR-363 in 293 cells transfected with plasmids carrying mutant SS6Mut or DSMut pre-miR-363 sequence. (**P<0.001). F. The impact of mutant SS6Mut or DSMut pre-miR-363 on suppression of cell invasion in ACHN cells (*P<0.05, ***P<0.00001). G. Expression level of Slug mRNA in 293 cells transfected with plasmids carrying mutant SS6Mut or DSMut pre-miR-363 sequence. (**P<0.001).