Fig. 3-. The position of the abasic site on the G4 influences Ape1 endonuclease activity.

A) Representative denaturing polyacrylamide gels of AP site incision by Ape1^WT on S4 and S8 incubated with the indicated amount of protein in a solution containing 50 mM KCl as described in Materials and methods section (left).

B) Representative denaturing polyacrylamide gels of AP site incision by Ape1^N∆33 on S4 and S8 incubated with the indicated amount of protein in a solution containing 50 mM KCl as described in Materials and methods section (left).

Graphs describe the percentage of conversion of substrate (S) into product (P) as a function of the dose of Ape1 protein on the specified substrate (right). ds-F ODN was used as positive control. S denotes the substrate position; the length of the generated products is 1 nt different between S8 (P) and ds-F (P’). Average values are plotted with standard deviations of three loadings of the same experiment as a function of protein dosage. Standard deviation values were always less than 10% of the mean of experimental points. See also supplementary Fig. S5A and S5B for the gels.