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. 2019 Feb;10(1-2):39–51. doi: 10.18632/genesandcancer.185

Figure 4. Effects of chronic, low dose sodium arsenite treatment on mitotic regulators.

Figure 4

A. The phosphorylated BubR1 and Cdc27 in the cells, after releasing from nocodazole block, were analyzed by immunoblotting analysis. B. After blocking protein synthesis by CHX, the levels of cln B1 expression in the cells were tested at different time points of nocodazole releasing. C. After adding actinomycin D (ATC) to block DNA synthesis, the cells were synchronized in mitosis by nocodazole treatment. Following releasing from mitosis block, quantitative PCR was performed to determine the levels of cln B1 expression. Error bars represent SD from 5 experiments (p < 0.05).