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. Author manuscript; available in PMC: 2019 Mar 16.
Published in final edited form as: Cell Rep. 2017 May 9;19(6):1189–1201. doi: 10.1016/j.celrep.2017.04.031

Figure 3. Transient Luciferase Reporter Assays and ChIP Analysis for the PD-L1 Promoter in M381 Melanoma Cells.

Figure 3

(A) Sequence of the PD-L1 promoter showing the position of the most representative putative binding sites of the promoter, STAT1/STAT3, STAT2/STAT5, and IRF1.

(B) PD-L1 promoter transient reporter assay including deletions of the putative binding sites. Results are represented as normalized relative luciferase units (RLUs).

(C) ChIP assay in M381 cells at the PD-L1 promoter (gray), the HLA-B promoter as a positive control (white), and the human tRNA-Leu anti-codon (TAG) as irrelevant sequence for IRF1 binding (negative control). Results are represented as percent enrichment relative to input. Asterisks denote significance in an unpaired t test (*p < 0.005, **p < 0.001), and error bars denote SD.