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. Author manuscript; available in PMC: 2019 Dec 1.

Published in final edited form as: DNA Repair (Amst). 2018 Sep 20;72:56–63. doi: 10.1016/j.dnarep.2018.09.008

Fig. 4. — Single-turnover kinetics of TDG^FL and TDG^82-308 excising T from a T:G mismatch in an NCP. Kinetic time courses were used to evaluate the excision of T in NCP substrates in a 5'-TpG/CpG-3' sequence context. Reactions consisted of 20 nM DNA substrate, 400 nM TDG^FL and TDG^82-308 in Buffer TNK plus 4% (v/v) glycerol. Data were fit to a single exponential equation. Error bars represent standard error (n=3 for TDG^FL; n=4 for TDG^82-308). Blue diamonds (), TDG^FL; green triangles (), TDG^82-308. The observed rates (fraction product) are k_obs=0.3 ± 0.1 min⁻¹ (0.03) (TDG^FL) and k_obs=0.5 ± 0.1 min⁻¹ (0.10) (TDG^82-308).

Inline graphic — Single-turnover kinetics of TDG^FL and TDG^82-308 excising T from a T:G mismatch in an NCP. Kinetic time courses were used to evaluate the excision of T in NCP substrates in a 5'-TpG/CpG-3' sequence context. Reactions consisted of 20 nM DNA substrate, 400 nM TDG^FL and TDG^82-308 in Buffer TNK plus 4% (v/v) glycerol. Data were fit to a single exponential equation. Error bars represent standard error (n=3 for TDG^FL; n=4 for TDG^82-308). Blue diamonds (), TDG^FL; green triangles (), TDG^82-308. The observed rates (fraction product) are k_obs=0.3 ± 0.1 min⁻¹ (0.03) (TDG^FL) and k_obs=0.5 ± 0.1 min⁻¹ (0.10) (TDG^82-308).