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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Cancer Res. 2019 Jan 23;79(6):1165–1177. doi: 10.1158/0008-5472.CAN-18-0921

Figure 3.

Figure 3.

AML cells treated dmPGE2 or co-cultured with MSCs express increased β-catenin and ARC and are more resistance to chemotherapy. A. OCI-AML3 cells were treated with dmPGE2 (1 or 4 μM) or co-cultured with MSCs without or with Cox-2 inhibitor Celecoxib (200 nM) for 48 h. β-catenin and ARC protein levels were determined by western blot. For co-culture experiments, OCI-AML3 cells were FACS-sorted after co-culture and west blot was done using the lysate from the sorted CD45+CD90− cells as shown in the histogram. B. OCI-AML3 cells were treated with dmPGE2 (4 μM) or co-cultured with MSCs for 48 h. Cytosolic and nuclear β-catenin levels were determined by western blot. C. OCI-AML cells were treated with Ara-C with or without dmPGE2 (1 or 4 μM) or with MSC co-culture in the absence or presence of Cox-2 inhibitor Celecoxib (200 nM) for 48 and 72 h. Apoptosis was assessed by flow cytometry. COX, co-culture.