Figure 4. Single cell transcriptomics of T_H17 cells and mTORC1-dependent licensing for T_H1 transdifferentiation.

a, Two-dimensional tSNE projection of single cell transcriptomics data from WT (black) and Rptor^Il17aCre (red) YFP⁺ cells (n = 3 per genotype; 27,619 total cells). b, tSNE visualization of gene signatures of upregulated and downregulated genes shared between memory CD8⁺ and T_FH cells (Choi et al.). c, tSNE visualization of “early memory” and “late memory” gene signatures (Wirth et al. and Muranski et al.). d, Pseudotime trajectory of single cell transcriptomics data colorized by pseudotime (arbitrary units; blue and red indicate early and late time, respectively). e, Pseudotime trajectory of cellular density: WT (gray), Rptor^Il17aCre (red). f, g, Cytokine production (f) and T-bet and TCF-1 expression (g) by CD27⁺ and CD27⁻ YFP⁺ cells cultured with MOG or MOG+IL-12 for 4 days. Data are representative of one (a–e) or three (f, g) independent experiments.