Table 1.

Comparison of GI screen methods

Method	Available systematic strain collections	Available systematic plasmid libraries	Time to combine 2 allelesa	Time to combine 3 allelesa	Types of GIsb	Selection method	Plasmids required
OE plasmid	Yesc	Yesg	3 days	N/A	DS, DE	2 markers	Episomal
SGA	Yesd	Yesg	17 days	34 days	DS, DE, SS, SE	2 + markers	Noi
dSLAM	Yesd	No	11 days	N/A	DS, DE, SS, SE	2 markers	YIp
Green Monster	Noe	N/A	11 days	22 days	SS, SE	GFP intensity	No
CRISPR/Cas	Yesf	Yesh	7 days	7 days	DS, DE, SS, SE	2 + markers	Episomal

^aStarting with single-gene alleles. Not including genotyping of strains. Approximate time given in days

^bDS dosage suppression, DE dosage enhancement including synthetic dosage lethality, SS synthetic suppression, SE synthetic enhancement including synthetic lethality

^cSopko et al. (2006), Douglas et al. (2012), Youn et al. (2017), also deletion strain collections can be used in conjunction with a query plasmid

^dGiaever et al. (2002)

^eGiaever et al. (2002) the haploid KO collection is used as the base strain, but the kanMX cassettes must be converted to the Green Monster GFP cassette

^fSmith et al. (2017)

^gZhu et al. (2001), Gelperin et al. (2005), Moriya et al. (2006), Hu et al. (2007), Magtanong et al. (2011)

^hSmith et al. (2016), Chen et al. (2017), Guo et al. (2018), Sadhu et al. (2018)

ⁱFor dosage screens, the query strain can carry an episomal OE plasmid or an integrated OE cassette