Table 2.
COX17, COX19 and COX23 mRNA half-lives were measured in isogenic wild-type (UPF1 rpb1-1) and NMD mutants (upf1Δ rpb1-1)
| Growth media | Yeast strain | COX17 mRNA | COX19 mRNA | COX23 mRNA |
|---|---|---|---|---|
| YAPD | UPF1 | 6.3 ± 1.5 | 5.94 ± 1.44a | 16.19 ± 2.43a |
| YAPD | upf1∆ | 17 ± 4 | 47.48 ± 22.51a | 12.20 ± 2.05a |
| Low Cu | UPF1 | 6.7 ± 0.6 | 3.7 ± 0.6 | 7.7 ± 4.0 |
| Low Cu | upf1∆ | 7.5 ± 1.0 | 14.7 ± 4.2 | 7 ± 2.6 |
| 100 µM Cu | UPF1 | 12.3 ± 4.7 | 4.0 ± 1.0 | 10.7 ± 4.5 |
| 100 µM Cu | upf1∆ | 13 ± 3.0 | 12.3 ± 0.57 | 9.6 ± 4.7 |
All yeast strains used were grown under the conditions indicated in the table
ND not determined
aHalf-lives were reported previously (Peccarelli et al. 2016)