Figure 2.

Treatment with PNA reduces intracellular reactive oxygen species. 4T1 cells were treated with PNA or corresponding concentration of control HSA for 16-18 hours and then stained with 2',7'-dichlorofluorescein diacetate and measured via flow cytometry. In the presence of reactive oxygen species (ROS), the dye is converted to the fluorescent molecule 2',7'-dichlorofluorescein (DCF) (n=3 for each group; data is representative of three independent experiments; ∗ = p < 0.05, ∗∗ = p < 0.01 versus HSA).